Journal: The Journal of nutritional biochemistry

Article Title: Dietary polyunsaturated fatty acids modulate adipose secretome and is associated with changes in mammary epithelial stem cell self-renewal

doi: 10.1016/j.jnutbio.2019.05.007

Figure Lengend Snippet: Dietary fatty acids influence adipose tissue macrophage polarization. (A) The CD68/FABP4 ratio in total adipose tissue lysate. There was no statistical difference in macrophage population in the two diet groups (P=.31). (B) The macrophage M1/M2 polarization ratio was estimated by the iNOS/Arg1 ratio in total adipose tissue lysate. Animals in the fish oil diet had a 58% reduction in the M1/M2 polarization ratio compared to animals in the Western blend diet group (0.67 and 0.28, respectively). *P≤.05.

Article Snippet: Pelleted Western blend control diet (ω-3:ω-6 fatty acid ratio=0.05) and menhaden fish oil diets (ω-3:ω-6 fatty acid ratio=0.4) were prepared by Dyets Inc. (Bethlehem, PA, USA).

Techniques: Western Blot

Journal: The Journal of nutritional biochemistry

Article Title: Dietary polyunsaturated fatty acids modulate adipose secretome and is associated with changes in mammary epithelial stem cell self-renewal

doi: 10.1016/j.jnutbio.2019.05.007

Figure Lengend Snippet: Adipose-derived factors differentially modulate human mammary stem cell self-renewal. (A) Pooled mammary epithelial cells were grown in nonadherent cultures to form mammospheres and treated with adipose tissue conditioned media from rats fed either fish oil or the Western diet, or negative control media. Primary MFE was unchanged between diet groups and the vehicle control, when normalized to the spheres treated with the negative control media (M199+1 mg/ml BSA). (B) Primary mammospheres were dissociated and replated to form secondary mammospheres as a measure of stem cell self-renewal. The change in secondary mammospheres over primary mammospheres was normalized to the negative control; graph shows the mean % change±S.E.M. Factors present an adipose conditioned media from animals fed the standard Western Blend diet increased the rate of stem cell self-renewal by 61.6%±9.03% (X¯%±S.D.) compared to the negative control (P<.001), while factors in the adipose secretome from animals fed a fish oil-supplemented diet attenuated this effect with a mean increase in stem cell self-renewal of only 11.4%±10.59% compared to the negative control (P=.12).*P≤.05.

Article Snippet: Pelleted Western blend control diet (ω-3:ω-6 fatty acid ratio=0.05) and menhaden fish oil diets (ω-3:ω-6 fatty acid ratio=0.4) were prepared by Dyets Inc. (Bethlehem, PA, USA).

Techniques: Derivative Assay, Western Blot, Negative Control, Control